First time I am working with NGS data. I've got a BAM file with mapped reads for my samples and a BED file with the regions in HG19 that were targeted (used an Ion-torrent ampliseq panel). Are there any tools that can output something similar to this:
**Sample Amplicon Chromosome Start_coordinate_of_coverage End_coordinate_of_coverage**
Sample1 amp_001 chr6 1,000,000 1,000,250
Sample2 amp_001 chr6 1,000,111 1,000,255
Sample1 amp_002 chr6 1,000,200 1,000,333
I basically want to know for each gene what coverage we have for each sample.
EDIT: changed column headings, I'm looking for coordinates that have coverage, not depth at each exon.